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example 1  (Sino Biological)


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    Structured Review

    Sino Biological example 1
    Example 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b7+h3/Human+B7-H3+%2F+CD276+Protein/us12606625-259-8-19
    Average 94 stars, based on 16 article reviews
    example 1 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression.
    Article Snippet: .. To demonstrate the effect of soluble B7-H3 on VEGFA expression, cell culture supernatants of CRC cells treated with or without 50 ng/ml recombinant human B7H3 (rB7-H3, Sino Biological, Beijing, China, #11188- H02H) for 24 h were collected. ..

    Article Title: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression
    Article Snippet: The protein levels of soluble B7-H3 (sB7-H3), VEGFA, fibroblast growth factor 2 (bFGF) and platelet-derived growth factor-BB (PDGF-BB) in cell culture supernatants were measured with ELISA kits (Bright Scistar Biotechnology Co., Ltd., Suzhou, China, #XG-K3004 for soluble B7-H3; NeoBioscience, Shenzhen, China, #EHC108 for VEGFA, #EHC130 for bFGF, and #EHC181 for PDGF-BB) according to the manufacturer’s instructions. .. To demonstrate the effect of soluble B7-H3 on VEGFA expression, cell culture supernatants of CRC cells treated with or without 50 ng/ml recombinant human B7-H3 (rB7-H3, Sino Biological, Beijing, China, #11188-H02H) for 24 h were collected. ..

    Cell Culture:

    Article Title: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression.
    Article Snippet: .. To demonstrate the effect of soluble B7-H3 on VEGFA expression, cell culture supernatants of CRC cells treated with or without 50 ng/ml recombinant human B7H3 (rB7-H3, Sino Biological, Beijing, China, #11188- H02H) for 24 h were collected. ..

    Article Title: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression
    Article Snippet: The protein levels of soluble B7-H3 (sB7-H3), VEGFA, fibroblast growth factor 2 (bFGF) and platelet-derived growth factor-BB (PDGF-BB) in cell culture supernatants were measured with ELISA kits (Bright Scistar Biotechnology Co., Ltd., Suzhou, China, #XG-K3004 for soluble B7-H3; NeoBioscience, Shenzhen, China, #EHC108 for VEGFA, #EHC130 for bFGF, and #EHC181 for PDGF-BB) according to the manufacturer’s instructions. .. To demonstrate the effect of soluble B7-H3 on VEGFA expression, cell culture supernatants of CRC cells treated with or without 50 ng/ml recombinant human B7-H3 (rB7-H3, Sino Biological, Beijing, China, #11188-H02H) for 24 h were collected. ..

    Recombinant:

    Article Title: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression.
    Article Snippet: .. To demonstrate the effect of soluble B7-H3 on VEGFA expression, cell culture supernatants of CRC cells treated with or without 50 ng/ml recombinant human B7H3 (rB7-H3, Sino Biological, Beijing, China, #11188- H02H) for 24 h were collected. ..

    Article Title: Engagement of CD300c by a novel monoclonal antibody induces the differentiation of monocytes to M1 macrophages.
    Article Snippet: Data were analyzed using CytExpert (Beckman Coulter). .. Plates were coated with 800 ng/mL human CD300c recombinant protein, human PD-L1, PD-L2, B7-H2(ICOS-L), B7-H3, B7-H4, B7-1 (CD80), and B7-2 (CD86) (Sino Biological, Beijing, China) at 4 ◦C overnight. .. After blocking with skim milk, the plates were incubated with eight different concentrations of CL7 (10-fold in the range of 0.001–––10 μg/mL) for 1 h. After washing the wells, 100 μL of horse radish peroxidase (HRP)-conjugated anti-human IgG (Sigma-Aldrich) was added to each well, and then incubated for another 1 h. After washing with phosphate-buffered saline/0.1 % Tween (PBST), the plates were incubated with 3,3′,5,5′-tetramethylbenzidine solution, and 1 N H2SO4 was added to stop the reaction.

    Article Title: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression
    Article Snippet: The protein levels of soluble B7-H3 (sB7-H3), VEGFA, fibroblast growth factor 2 (bFGF) and platelet-derived growth factor-BB (PDGF-BB) in cell culture supernatants were measured with ELISA kits (Bright Scistar Biotechnology Co., Ltd., Suzhou, China, #XG-K3004 for soluble B7-H3; NeoBioscience, Shenzhen, China, #EHC108 for VEGFA, #EHC130 for bFGF, and #EHC181 for PDGF-BB) according to the manufacturer’s instructions. .. To demonstrate the effect of soluble B7-H3 on VEGFA expression, cell culture supernatants of CRC cells treated with or without 50 ng/ml recombinant human B7-H3 (rB7-H3, Sino Biological, Beijing, China, #11188-H02H) for 24 h were collected. ..

    Article Title: B7-H3-Targeting Chimeric Antigen Receptors Epstein Barr Virus Specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3 Positive Solid Tumors
    Article Snippet: .. B7-H3–specific VHHs To construct the VHH library, a naïve male llama was immunized with recombinant human B7-H3 (Sino Biological) at 100 μg per injection for a total of 6 injections. ..

    Construct:

    Article Title: B7-H3-Targeting Chimeric Antigen Receptors Epstein Barr Virus Specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3 Positive Solid Tumors
    Article Snippet: .. B7-H3–specific VHHs To construct the VHH library, a naïve male llama was immunized with recombinant human B7-H3 (Sino Biological) at 100 μg per injection for a total of 6 injections. ..

    Injection:

    Article Title: B7-H3-Targeting Chimeric Antigen Receptors Epstein Barr Virus Specific T Cells Provides a Tumor Agnostic Off-The-Shelf Therapy Against B7-H3 Positive Solid Tumors
    Article Snippet: .. B7-H3–specific VHHs To construct the VHH library, a naïve male llama was immunized with recombinant human B7-H3 (Sino Biological) at 100 μg per injection for a total of 6 injections. ..



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    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, <t>anti-B7-H3</t> CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
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    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, <t>anti-B7-H3</t> CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
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    Image Search Results


    A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Journal: bioRxiv

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    doi: 10.64898/2026.04.15.718753

    Figure Lengend Snippet: A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.

    Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

    Techniques: Immunocytochemistry, Expressing, Fluorescence, Co-Culture Assay

    Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Journal: bioRxiv

    Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors

    doi: 10.64898/2026.04.15.718753

    Figure Lengend Snippet: Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.

    Article Snippet: Primary human anti–B7-H3 antibody (AF1027, R&D Systems) was diluted in cold staining buffer (0.5 μg/μL; 2 μL per 100 μL reaction volume) and applied to cells for 20–30 min at 4 °C.

    Techniques: Activity Assay, Imaging